Application of Hydrocyclone for Cell Separation in Mammalian Cell …
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Numerical simulation of hydrocyclones for cell separation
According to the present work, Bradley hydrocyclones with diameters down to 10 mm cannot efficiently separate microorganisms, but the separation of mammalian cells with predicted efficiencies as high as 90% can be achieved. Numerical simulation of hydrocyclones aiming at investigating the separation of microorganisms and …
Numerical simulation of hydrocyclones for cell separation
Numerical simulation of hydrocyclones aiming at investigating the separation of microorganisms and mammalian cells was performed using Computational Fluid Dynamics (CFD). The turbulence model used in the 2d- axisymmetric calculations was the Reynolds. ... Numerical simulation of hydrocyclones for cell separation.
Hydrocyclones as cell retention device for CHO perfusion …
Such investigation has demonstrated that hydrocyclones are promising in separating mammalian cells, with cell separation efficiency at 81 % for one hydrocyclone (94% for two in series) and cell ...
Application of Hydrocyclone for Cell Separation in Mammalian Cell …
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Separation of CHO cells using hydrocyclones
The best performance was obtained with a 10-mm Dorr-Oliver hydrocyclone, resulting in a separation efficiency of 81%. These experimental values …
Feed Flow Pulsation in the Separation of CHO Cells in
Perfusion cultivations of animal cells present many advantages over batch and fed-batch cultivations (Castilho and Medronho 2002).Recently, the application of hydrocyclones for mammalian cell retention has been evaluated (Deckwer et al. 2005; Elsayed et al. 2006; Pinto et al. 2008).Hydrocyclones are characterized by their small …
Acoustic Settler, Centrifuge, Hydrocyclone
A detailed description of the hydrocyclone for mammalian cell culture can be found in Castilho's review (Castilho and Medronho 2002). Hydrocyclones are often used to separate solids from liquids or liquids with different densities in chemical processes. The device allows continuous cell separation and has the advantage of not including any ...
Application of hydrocyclones for continuous cultivation of SP-2/0 cells
The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors. 2013 • ... During the last two decades, they have been evaluated for their possible application in the separation of microbial and mammalian cells. Nowadays, mammalian cells are widely used for the production of a large number of ...
Application of Hydrocyclone for Cell Separation in …
produced by mammalian cell systems. Mammalian cells have the advantage of precisely producing recombinant proteins with the same authentic protein folding structure as those produced in human bodies. Developments achieved in the different cultivation systems have accelerated the progress of mammalian cell culture. Generally, large-scale ...
Data Hydrocyclone SBL2001-e
Hydrocyclone and one for the return flow (Underflow). The sterile connection of the. pre-sterilized bioreactor and Hydrocyclone. ... Mammalian cell retention device for single-use and reusable bioreactors. Perfusion Technologies. Overflow (Harvest) Underflow (Concentrate) Feed Feed.
Application of Hydrocyclone for Cell Separation in Mammalian Cell …
Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures (English shop) Elsayed Ahmed Elsayed Ahmed (Author) Preview. Table of Contents, Datei (88 KB) Extract, Datei (160 KB) ISBN-13 (Printausgabe) 3865376347: ISBN-13 (Hard Copy) 9783865376343: ISBN-13 (eBook) 9783736916340: Language: Alemán: Page Number: …
Hydrocyclone and N‐1 perfusion bioreactor performance …
Using a two-step micro-engineered system combining hydrocyclone with spiralmicrochannels for cell retention, a recent study has reported an impressive high cell density of 80 × 10 6 cells/mL [43].
Use of Hydrocyclones for Mammalian Cell Retention: …
A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation …
The Potential of Hydrocyclone Application for Mammalian Cell …
DOI: 10.6000/1927-3037.2013.02.04.2 Corpus ID: 84350518; The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors @article{Elsayed2013ThePO, title={The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors}, author={Elsayed …
The Use of Hydrocyclones for Mammalian Cell Retention in …
Abstract. A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell separation efficiencies as high as …
Use of Hydrocyclones for Mammalian Cell …
The cell viability was insignificantly affected due to the short residence time inside the hydrocyclone (0.03–0.1 s). The separation efficiency increased to 94 % when they connected two hydrocyclones in a series. Jockwer et …
Emerging application of hydrocyclone in biotechnology and …
The processing of bioproducts and food often requires efficiently separating particles such as mammalian or yeast cells from solutions. The rapid growth of biotechnology and food sectors has attracted increasing attention to exploring hydrocyclones as efficient separation instruments because they can operate …
Hydrocyclones as cell retention device for CHO perfusion
Abstract. In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of …
The Use of Hydrocyclones for Mammalian Cell Retention in …
A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell separation efficiencies as high as 99%.
Use of Hydrocyclones for Mammalian Cell Retention: …
The results indicate that the hydrocyclone can be appropriately used for cell retention and separation in perfusion cultures. A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops …
The Use of Hydrocyclones for Mammalian Cell …
The cell suspension (5 x 105 to 1.0 x 106 mL -1) was then pumped through each of the nine hydrocyclone configurations at pressure drops of 1 (Table I) and 1.35 bar (data not shown), respectively. At the inlet and outlets of the hydrocyclone, flow rate, cell concentration, and cell size distribution were measured for all nine combinations.
The Use of Hydrocyclones for Mammalian Cell Retention in …
A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes and gave cell separation efficiencies as high as 99%, witility always above 90% during the 23 days run. A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell …
Experimental and numerical analysis of Chinese hamster ovary cell …
Preservation of cellular viability is one key consideration in the hydrocyclone process, particularly for fragile cells such as mammalian cells that lack a cell wall [34]. ...
Use of Hydrocyclones for Mammalian Cell …
A hydrocyclone with a volume of 2.56 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to …
Use of Hydrocyclones for Mammalian Cell Retention: …
A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow ...
Numerical Simulation of Hydrocyclones for Cell Separation
Cultures of BHK and HeLa cells (with low cell concentrations) were applied to measure separation efficiency and cell viability for a hydrocyclone operation period of 3 min corresponding to a cell ...
Application of Hydrocyclone for Cell Separation in …
4.1.2. Effect of hydrocyclone geom etry on the physical characterist ics of separation. 51 4.2. Effect of different pressures gen erated by th e hydrocyclone on cell viability and the separation efficiency of the hydrocyclone..... 55 4.2.1. BHK cells ..
Advantages of Hydrodynamic Cell Separation In Industrial Cell …
Jockwer et al. (2001) investigated nine different hydrocyclone geome- tries for mammalian cell separation. With their optimum configuration they were able to cultivate continuously CHO cells for 23 days with a viability range above 90%. Furthermore, Elsayed et al. (2006) showed cell separation efficiencies of up to 0.97 without any loss in ...
Use of Hydrocyclones for Mammalian Cell Retention: …
A hydrocyclone with a volume of 2.56 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow-to-overflow ratio) and flow ratio …